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Nanochitosan antimicrobial activity against Streptococcus mutans and Candida albicans dual-species biofilms
Ikono R.a,b,c, Vibriani A.d, Wibowo I.d, Saputro K.E.a, Muliawan W.a, Bachtiar B.M.e, Mardliyati E.f, Bachtiar E.W.e, Rochman N.T.g, Kagami H.h, Xianqi L.h, Nagamura-Inoue T.c, Tojo A.c
a Division of Bionanotechnology, Nano Center Indonesia, Tangerang Selatan, Indonesia
b Department of Metallurgical Engineering, Sumbawa University of Technology, Sumbawa Besar, Indonesia
c Division of Molecular Therapy, Institute of Medical Science, University of Tokyo, Tokyo, Japan
d School of Life Science and Technology, Bandung Institute of Technology, Bandung, Indonesia
e Oral Science Laboratory, Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
f Center for Pharmaceutical and Medical Technology, Agency for the Assessment and Application of Technology BPPT, Tangerang Selatan, Indonesia
g Research Center for Physics, Indonesian Institute of Science LIPI, Tangerang Selatan, Indonesia
h Department of Oral and Maxillofacial Surgery, Matsumoto Dental University, Shiojiri, Japan
i Department of General Medicine, IMSUT Hospital, Institute of Medical Science, University of Tokyo, Tokyo, Japan
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2019 The Author(s).Objective: Chitosan nanoparticle (nanochitosan) has a broad antimicrobial spectrum against diverse pathogenic microorganisms. However, its effect on dental caries-associated microorganisms, such as Streptococcus mutans and Candida albicans is yet to be explored. These microorganisms are known for causing early childhood caries. Therefore, this study was aimed at investigating nanochitosan inhibition capacity against dual-species biofilms of S. mutans and C. albicans. In this study, nanochitosan antimicrobial activity is reported against mono and dual biofilm species of S. mutans and/or C. albicans at 3 and 18 h incubation time. Nanochitosan inhibition capacity was observed through biofilm mass quantity and cell viability. Results: The present study successfully synthesized nanochitosan with average diameter of approximately 20-30 nm, and also established dual-species biofilms of S. mutans and C. albicans in vitro. With nanochitosan treatment, the cell viability of both microorganisms significantly decreased with the increasing concentration of nanochitosan. There was no significant decrease in biofilm mass both in the dual and single-species biofilms after 3 h of incubation. However, greater inhibition of biofilm was observed at 18 h incubation.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Anti-Infective Agents,Biofilms,Candida albicans,Child,Chitosan,Dental Caries,Dental Plaque,Humans,Microbial Viability,Microscopy, Electron, Transmission,Nanoparticles,Particle Size,Streptococcus mutans,Time Factors[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Biofilm,Candida albicans,Caries,Nanochitosan,Streptococcus mutans[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]This research was funded by Japan Society for the Promotion of Science [JSPS] [ID No: R11525] by giving the funding for the design of study and data collection. Besides, the financial support was also provided by Grant‑in‑Aid for Scientific Research [B] [JSPS Kakenhi Grant Number JP16H05546] by giving the funding for data analysis and interpretation.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.1186/s13104-019-4422-x[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]