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Biosynthesis and characterization of bioplastic polyhydroxybutyrate from halophilic bacterium Halomonas elongata BK-AB8
Rachmawati R.a, Falahuddin W.a, Hertadi R.a
a Inorganic and Physical Chemistry Division, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Bandung, 40132, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2019 Trans Tech Publications Ltd, Switzerland.Bioplastics are biodegradable polymers which can be produced by bacteria. The production occurs when the bacteria grow in a medium containing a minimum amount of key nutrients, such as nitrogen, magnesium, and phosphorus but is rich of carbon source. It is known that some bacteria from the genus Halomonas can produce bioplastics compound such as polyhydroxybutyrate (PHB). This study focused to evaluate the potential of Halomonas elongata BK-AB8 in producing PHB. The bacteria were grown on a medium containing nile red. The grown bacterial colony exhibited orange luminescence when exposed under UV light indicating that the bacteria was able to produce bioplastics. The bioplastic production was conducted by growing the bacteria in the modified growth medium at 37 °C for 22 hours with an aeration rate of 150 rpm. The resulting bioplastic was extracted using a mixture of chloroform-NaOCl (1:1), and subsequently precipitated in methanol. The highest efficiency of bioplastics production by Halomonas elongata BK-AB8 was around 24.7% using glucose as the main carbon source. 1H-NMR, 13C-NMR, and FTIR characterizations of the resulting bioplastic showed high similarities to the corresponding profiles of PHB, which is likely in the form of poly-(R)-hydroxybutyrate. Thermogravimetry Analysis (TGA) showed that the resulting PHB sample started to degrade at 210 °C. Further characterization by Differential Scanning Calorimetry (DSC) showed a melting temperature of PHB at 164 °C with a degree of crystallinity around 14.2%, which strongly indicates that PHB was successfully produced from Halomonas elongata BK-AB8.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Bioplastics,Bioplastics production,Degree of crystallinity,FT-IR characterization,Halomonas elongata,Halophilic bacteria,Polyhydroxybutyrate,Thermogravimetry analysis[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Biodegradable polymer,Bioplastic,Halomonas elongata,Polyhydroxybutyrate[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]This research was supported by the ITB research grant (project number 6431/I1.B04/PL/2016) and the research grant from the Ministry of Research, Technology and Higher Education (project number 077/SP2H/LT/DRPM/II/2016).[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.4028/www.scientific.net/KEM.811.28[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]