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Poly-β-Hydroxybutyrate (PHB) Improves Nursery-Phase Pacific White Shrimp Litopenaeus vannamei Defense against Vibriosis
Situmorang M.L.a, Suantika G.a, Santoso M.a, Khakim A.a, Wibowo I.a, Aditiawati P.a, Haniswitaa
a Microbial Biotechnology Research Group, School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung, 40132, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2019 American Fisheries SocietyThis study assessed the effect of poly-β-hydroxybutyrate (PHB) on the growth, survival, and disease resistance of Pacific white shrimp Litopenaeus vannamei. A 20-d feeding test using 30-d-postlarval Pacific white shrimp (100 shrimp/tank) tested three different diets: 0.3 g PHB/kg diet, 0.5 g PHB/kg diet, and commercial feed containing no PHB as the control. The feeding test was followed by a 10-d bacterial challenge test using Vibrio harveyi. After the feeding test, the highest average final body weight, specific growth rate, and total biomass (mean ± SD = 0.44 ± 0.28 g, 28.82 ± 2.08%/d, and 25.91 ± 7.19 g, respectively) were found in the 0.5 g PHB/kg diet group, while the 0.3 g PHB/kg diet showed the highest survival rate (67.00 ± 8.83%). After the Vibrio harveyi challenge test, both PHB diet treatments resulted in the same significantly higher survival rate (96.66 ± 4.71%) compared with the control treatment (70.00 ± 4.71%). The highest body weight gain during the challenge test was seen in the 0.5 g PHB/kg diet group. Total abundance of heterotrophic bacteria and Vibrio spp. was relatively similar for all treatments. Histopathological analysis showed that hepatopancreas samples from PHB-supplemented Pacific white shrimp showed decreasing tubular epithelial cell lesions as a result of Vibrio harveyi infection, suggesting that PHB protected the hepatopancreas of Pacific white shrimp from the destructive effect of Vibrio harveyi infection. Supplementation at 0.5 g PHB/kg significantly increased Pacific white shrimp survival and growth with or without Vibrio harveyi infection and, thus, has high potential for further use in Pacific white shrimp aquaculture.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][{‘$’: ‘This research was supported by Riset ITB 2016, Institut Teknologi Bandung, Indonesia. There is no conflict of interest declared in this article.’}, {‘$’: ‘This research was supported by Riset ITB 2016, Institut Teknologi Bandung, Indonesia. There is no conflict of interest declared in this article.’}][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.1002/naaq.10132[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]