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The use of PCR and BsmAI restriction combination targeting wciP gene to determine serotype 6A, 6B, 6C and 6D Streptococcus pneumoniae
Tafroji W.a, Bernadette F.M.b, Giri Rachman E.A.b, Safari D.a
a Molecular Bacteriology Laboratory, Eijkman Institute for Molecular Biology, Indonesia
b School of Life Sciences and Technology (SITH), Bandung Institute of Technology (ITB), Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2020 Elsevier B.V.We designed a sensitive and reliable method to distinguish serogroup 6 using PCR followed by enzymatic restriction digest. We discovered that this serotyping method was able to distinguish serotypes 6A, 6B, 6C, and 6D based on the recognition site of BsmAI in the wciP region.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Bacterial Typing Techniques,Deoxyribonucleases, Type II Site-Specific,Genes, Bacterial,Polymerase Chain Reaction,Sensitivity and Specificity,Sequence Analysis, DNA,Serogroup,Serotyping,Streptococcus pneumoniae[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][{‘$’: ‘This study was supported by the Ministry of Research, Technology and Higher Education, Republic of Indonesia. We thank Miftahuddin Majid Khoeri, Korrie Salsabila, Wisiva Tofriska, and Sukma Oktavianthi for laboratory technical supports and discussions. We also thank Dorothy Southern for support and evaluation during manuscript preparation.’}, {‘$’: ‘This study was supported by the Ministry of Research, Technology and Higher Education , Republic of Indonesia. We thank Miftahuddin Majid Khoeri, Korrie Salsabila, Wisiva Tofriska, and Sukma Oktavianthi for laboratory technical supports and discussions. We also thank Dorothy Southern for support and evaluation during manuscript preparation.’}][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.1016/j.mimet.2020.105909[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]