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Inability of polysaccharides of Spirulina platensis to protect hepatocyte cells line on Toxoplasma gondii infection in vitro
Afililla Z.a, Suwanti L.T.a, Puspitasari H.a, Suyono E.A.b, Budiman A.b, Koerniawan M.D.c, Siregar U.J.d
a Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, 60115, Indonesia
b Faculty of Biology, Universitas Gadjah Mada Yogyakarta, Indonesia
c School of Architecture, Institut Teknologi Bandung, Bandung, Indonesia
d Faculty of Forestry, Institut Pertanian Bogor, Bogor, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2009-2020, JGPT. All Rights ReservedToxoplasmosis is one zoonotic disease which caused by an obligate intracellular protrozoan parasite, Toxoplasma gondii. The parasite infects humans and warm-blooded animals (mammals and birds). The drug of choice against toxoplasmosis is combination between pyrimethamin with sulfadiazine, but both can induce side effects and toxic to host cell, so alternative therapeutic compounds are needed. Many reseachers have explored microalgae for food and medicine. One of the main components of Spirulina platensis that plays a role in enhanching immunity is polysaccharides. The present study investigated effect of Spirulina polysaccharides on Toxoplasma gondii infection in Vitro. Polysaccharides of S. platensis were extracted with hot water and alkhaline method. Extraction Rate (ER), sugar and protein levels were compared between the two methods. The Hepatocyte Cells Line (HCL) were infected with T. gondii and treated with polysaccharides of S. platensis. The parameters were Parasite burden and Viability Host Cell. The result shows that Extraction Rate, carbohydrate and protein levels were affected by extraction method. Carbohydrate level in polysaccharides with hot water extraction was higher than alkhaline extraction, on the contrary, ER and protein level were lower. Polysaccharides of S. platensis did not protect HCL infected with T. gondii. More than 5 mg/mL polysaccharides of S. platensis was toxic to host HCL.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Alkaline extraction,Hot water extraction,Polysaccharides,Spirulina platensis,Toxoplasma gondii[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][{‘$’: ‘The authors gratefully thank to Research and Innovation Institute Universitas Airlangga, for financial support through research grant: Indonesian Research Collaboration – World Class University 2019, No: 264/UN3.14/LT/2019 9. Mordue DG, Monroy F, Regina ML, Dinarello CA, Sibley LD (2001) Acute Toxoplasmosis Leads to Lethal Overproduction of Th1 Cytokines. The American Association of Immunologists, 167: 4574-4584.’}, {‘$’: ‘The authors gratefully thank to Research and Innovation Institute Universitas Airlangga, for financial support through research grant: Indonesian Research Collaboration – World Class University 2019, No: 264/UN3.14/LT/2019’}][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]