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The potential of clove essential oil microemulsion as an alternative biocide against pseudomonas aeruginosa biofilm
Purwasena I.A.a, Astuti D.I.a, Taufik I.a, Putri F.Z.a
a Department of Microbiology, School of Life Science and Technology, Institut Teknologi Bandung, Bandung, Lebak Siliwangi, 40132, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© The Author(s) 2020. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.Biocorrosion phenomenon is known to be detrimental in oil and gas industry. Synthetic biocides have been widely used to eliminate microorganisms which promote biocorrosion. However, these biocides are known to be toxic and harmful to the environment. One of the natural agents that potentially serves as an alternative for biocide is clove essential oil which has antimicrobial constituents and also have been shown to inhibit quorum sensing (QS) process of Pseudomonas aeruginosa. In this study, clove essential oil extracted from clove bud and stem were formulated into microemulsion and evaluated for its antibiofilm and anti-QS activity. To observe its antibiofilm activity, minimum inhibitory concentration (MIC), minimum biofilm inhibitory concentration (MBIC), and minimum biofilm eradicating concentration (MBEC) of clove bud and stem essential oils were determined by microdilution method in microtiter 96 well-plate. Anti-QS activity was observed by analyzing clove bud and stem essential oil effect against P. aeruginosa’s extracellular protease activity and swarming motility. The MIC value of clove bud and stem essential oils microemulsions were 0,33% and 0,5%, while MBIC50% values for clove bud and stem essential oil were 0,33% and 0.5%. However, MBEC value for both clove essential oil could not be determined. In addition, 0.66% clove bud essential oil and 1% clove stem essential oil successfully inhibited extracellular protease activity and swarming motility of P. aeruginosa by 32.06% and 45.56% for clove bud, and 31.43% and 45% for clove stem, respectively.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Biocorrosion,Clove essential oil,Eugenol,MBEC,MBIC,MIC[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][{‘$’: ‘This study was also supported by a grant from Bandung Institute of Technology on P \uef00MI’}, {‘$’: ‘This study was also supported by a grant from Bandung Institute of Technology on P3MI 2017 scheme.’}][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.22207/JPAM.14.1.27[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]