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Inhibitory activity of the active compound of ethyl acetate fraction of tamoenju (Hibiscus surattensis L.) leaves against α-glucosidase and dipeptidyl peptidase-4 enzymes
Yulieta,b, Sukandar E.Y.a, Budipramana K.c, Adnyana I.K.a
a Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, Bandung Institute of Technology, Bandung, 40132, Indonesia
b Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Tadulako University, Palu, 94118, Indonesia
c Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Surabaya, Surabaya, 60293, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© RASĀYAN. All rights reserved.The ethyl acetate fraction (EAF) of tamoenju (Hibiscus surattensis L.) leaves, used as a traditional antidiabetic agent in Central Sulawesi, has high inhibitory activity against α-glucosidase and dipeptidyl peptidase-4 (DPP-4). The aim of this study was to isolate the active compound of EAF that was carried out by step gradient polarity extraction, then separated and purified by chromatography. The structure was identified and characterized using NMR spectroscopy and LC-MS. α-glucosidase inhibitory activity was evaluated using the in vitro standard α-glucosidase inhibition test, while DPP-4 activity was evaluated by ex vivo DPP-4 inhibitor test using rat blood serum as the enzyme source. IC50 values were determined by nonlinear regression curve and fit using GraphPad Prism 8 and were expressed as mean ± SEM. In this research, we obtained the isolated compound was identified as kaempferol, which was isolated for the first time from this plant. The isolate exhibited potent α-glucosidase and DPP-4 inhibition with IC50 values of 27.78 ± 0.86 and 7.37 ± 0.06 µg/mL, respectively. Acarbose and sitagliptin as positive control had IC50 values of 17.80 ± 0.27 and 25.56 ± 0.43 µg/mL, respectively. The isolate level by LC-MS was estimated to be 182.23 mg/g isolate. Therefore, tamoenju leaves have great potential as functional foods and in the development of antidiabetic drugs.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Antidiabetic,Dipeptidyl peptidase-4,Hibiscus surattensis L.,Kaempferol,Tamoenju,α-Glucosidase[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]The authors would like to thank the Ministry of Research, Technology, and Higher Education of the Republic of Indonesia and Indonesia Endowment Fund for Education (LPDP) for the funding through the BUDI DN Scholarship with the cooperation contract number: PRJ-109/LPDP.4/2019. We also thank Editage (www.editage.com) for English language editing.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.31788/RJC.2020.1325607[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]