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Biosurfactants production using glucose and molasses as carbon sources by Azotobacter vinelandii and soil washing application in hydrocarbon-contaminated soil
Devianto L.A.a, Latunussa C.E.L.b, Helmy Q.b, Kardena E.b
a Department of Environmental Engineering, Faculty of Agricultural Technology, Universitas Brawijaya, Malang, Indonesia
b Department of Environmental Engineering, Institut Teknologi Bandung, Bandung, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© Published under licence by IOP Publishing Ltd.The purpose of this research was to understand the influence of carbon source variation in the biosurfactant production by Azotobacter vinelandii as well as its potential application on remediation of hydrocarbon-contaminated soil by soil washing. The growth microorganism, biosurfactant quantity in exopolysaccharide and fatty acid fraction were examined. The quality of biosurfactant was analysed by emulsification index. The results show that biggest production was occurred at the 48th hour with μmax1 = 0.416/hour, μmax2 = 0.093/hour and Ks1 = 6.55 g/L, Ks2 = 4.69 g/L for glucose and molasses substrate respectively. Biosurfactant product of Azotobacter vinelandii in glucose 1.5%, 2%, 2.5%, and 3% showed emulsification index as much as 100%. While, emulsification index of the biosurfactant produced with molasses reached 100% within 1%, 1.5%, and 2% molasse concentration. These results showed that molasses has potential utilization as carbon source in biosurfactant production. In soil washing application, Biosurfactant and Tween 80 were used as emulsifier to removed Total Petroleum Hydrocarbons (TPH) by contacting in conical flask at rotary shaker. The results showed that highest removal achieved by using biosurfactant up to 48.89% which occurred at 100 rpm for 40 minutes.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Acid fractions,Bio-surfactants,Biosurfactant production,Conical flasks,Contaminated soils,Emulsification index,Exopolysaccharides,Total petroleum hydrocarbons[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.1088/1755-1315/475/1/012075[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]