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Anti-fungal substances of Bacillus subtilis against Ganoderma boninense
Singhalath S.a, Aryantha I.N.P.a
a Microbial Biotechnology Research Group, School of Life Sciences and Technology, Institut Teknologi Bandung (ITB), Bandung, 40132, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2019, Universiti Sains Malaysia.Aims: To determine the optimum culture incubation time for β-glucanase and chitinase production by Bacillus subtilis as well as optimum pH and temperature condition for enzymatic activity against Ganoderma boninense. The suitable solvent (methanol, ethyl acetate or hexane) for the extraction of bacterial metabolites from B. subtilis were also determined. Methodology and results: In vitro antagonistic activity of antifungal metabolites derived from B. subtilis to inhibit the growth of G. boninense was evaluated based on time of culture incubation, extraction solvent of the metabolites, and enzymatic treatments conditions including pH and temperature. The results showed that β-glucanase could be optimally produced (with a specific activity 4.222 U/mg-protein) after 28 h of incubation. The optimum pH and temperature for the activity of β-glucanase were 7.5 and 45 °C respectively when 1% laminarin used as the substrate. B. subtilis showed optimum chitinase activity (0.0514 U/mL) after 8 h of incubation. Optimum pH and temperature of chitinase were at pH 6.0 and 40 °C, respectively using 1% colloidal chitin as the substrate. β-glucanase crude enzyme showed strongest antifungal activities against the mycelial growth of G. boninense better than crude enzyme of chitinase with an inhibition rate of 47.75% at 5 days of incubation. Furthermore, cultivation of B. subtilis over 48 h produced antifungal metabolites which could inhibit the growth of G. boninense the most. The best solvent to extract metabolite from B. subtilis was identified as ethyl acetate that rendered an inhibition value of 38.91%. Conclusion, significance, and impact of study: Bacillus subtilis could be a potential biological control agent against G. boninense.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Antifungal,Bacillus subtilis,Chitinase,Ganoderma boninense,Β-glucanase[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]The authors are grateful to Kemitraan Negara Berkembang (KNB) Scholarship for a technical support manager. I would also like to thank PL Astra Agro Lestari for research foundation and School of Life Science and Technology for student financial assistance.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.21161/mjm.191546[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]