[vc_empty_space][vc_empty_space]
Point mutation of ITS-nrDNA sequences as specific markers of three durian species: Durio zibethinus, D. kutejensis and D. lowianus
Santoso P.J., Pancoro A.b
a Indonesian Tropical Fruits Research Institute, Indonesian Agency for Agricultural Research and Development, Ministry of Agriculture, Solok, West Sumatra, 27301, Indonesia
b School of Life Science and Technology, Bandung Institute of Technology, Bandung, West Java, 40132, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2020 Published under licence by IOP Publishing Ltd.Molecular markers are considered more efficient tools than morphological markers for species identification. This research aimed to get specific molecular markers among three durian species Durio zibhetinus, D. kutejensis and D. lowianus using ITS-nrDNA sequences. A number of 139, 7 and 1 ITS sequences of D. zibethinus, D. kutejensis and D. lowianus, respectively, were used in this experiment. Each group of sequences was then aligned to make one consensus sequence. In order to identify the point mutations, the aligned sequences were cut with restriction enzyme in silico using Genious ver. 7 software. The simulations found that each consensus sequences has different point mutation forming different restriction site. Consensus sequences of D. zibethinus has EcoR1 site on base number 280, D. kutejensis has Ama871 site on base number 394 and D. lowianus has Aco1 site on base number 135. These signals could be used as specific markers for the three durian species.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Consensus sequence,In-silico,Its sequences,Molecular marker,Morphological markers,Point mutations,Restriction enzymes,Species identification[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Durio,ITS-nrDNA,point mutation,species markers[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]This research was funded by Indonesian Agency for Agricultural Research and Development, Ministry of Agriculture, Republic of Indonesia through Cooperation Scheme of KKP3T year 2013.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.1088/1755-1315/482/1/012020[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]