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Silica-supported carboxylated cellulose nanofibers for effective lysozyme adsorption: Effect of macropore size
Rahmatika A.M.a,b, Goi Y.c, Kitamura T.c, Morita Y.c, Iskandar F.d, Ogi T.a
a Department of Chemical Engineering, Graduate School of Engineering, Hiroshima University, Higashihiroshima, 739-8527, Japan
b Department of Bioresource Technology and Veterinary, Vocational College, Gadjah Mada University, 55281, Indonesia
c R&D Headquarters, DKS Co. Ltd., Kyoto, 601-8391, Japan
d Department of Physics, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Bandung, 40132, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2020 The Society of Powder Technology JapanEngineering hierarchical macro/mesoporous structures that offer an abundance of accessible binding sites are highly desirable in protein adsorption processes. However, numerous significant challenges remain. Herein, cellulose nanofiber (CNF)-loaded macroporous silica (CNF-MPS) particles were successfully synthesized with a high degree of accessible binding sites by tuning the macropore size of the silica particles and loading a highly carboxylated CNF via smart and rational design. The as-prepared CNF-MPS particles exhibited a high negative charge (~−59 mV) and excellent protein adsorption ability (>1000 mg/g) in 1500 mg/g as a result of improved interconnectivity between the channels. Here exposed macropores of >100 nm allows ingress of protein to the interior structure that houses an abundance of binding sites comprising the dispersed CNF. Additionally, the adsorption kinetics, thermodynamics, and isothermal parameters were studied to analyze the mechanism of lysozyme adsorption. The adsorption process is confirmed to occur spontaneously at any temperature with a pseudo-second-order model describing the kinetic model, and CNF deposition affecting the heterogeneity of the binding sites.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Adsorption capability,Adsorption kinetics,Adsorption process,Cellulose nanofibers,Interior structure,Lysozyme adsorption,Macroporous silica,Pseudo-second order model[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Cellulose nanofiber,Lysozyme,Macroporous particle,Pore size control,Protein adsorption[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]This work was supported by JSPS KAKENHI Grant Number 19H02500 . This work is partly supported by the Center for Functional Nano Oxide at Hiroshima University , JSPS Core-to-Core Program, The Information Center of Particle Technology, Japan, Hosokawa Powder Technology Foundation , The Mazda Foundation , and the Electric Technology Research Foundation of Chugoku . The authors would like to thank the Japanese Ministry of Education, Culture, Sports, Science, and Technology (MEXT) for providing a doctoral scholarship (A. M. R.). We thank Edanz Group ( www.edanzediting.com/ac ) for editing a draft of this manuscript.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.1016/j.apt.2020.05.021[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]