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Isolation and characterization of a surfactant-stable protease from halophilic bacteria chromohalobacter japonicus bk-ab18
Achmad D.I.a, Ihsanawatia, Hertadi R.a
a Biochemistry Research Division, Faculty of Mathematics and Natural Sciences, Bandung Institute of Technology, Bandung, 40116, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2020, Malaysian Society of Applied Biology. All rights reserved.The protease from Chromohalobacter japonicus BK-AB18 was produced by growing bacteria in a LB medium containing 5% casein and 5% NaCl. The crude protease was partially purified by three levels of ammonium sulfate concentration (ranges of 0–70%, 70–75% and 75–80%) and the highest specific activity was exhibited in the range of 75–80%. The enzyme has a relative molecular weight of 65 kDa. The protease in this fraction had the highest activity in the following optimum conditions: 7.5% NaCl, a pH of 9.0 and a temperature of 45°C. The activity of the enzyme at the optimum pH and temperature was enhanced by the addition of a Ca2+ ion, but its activity was significantly inhibited by EDTA, hence this enzyme is included as metalloenzyme. Interestingly, the protease activity increased when exposed to a concentration of 0.01% and 0.05% SDS, and was relatively stable in this solution up to a concentration of 10%. It is thus demonstrated that C. japonicus BK-AB18 is a potential source to produce extracellular protease that can be applied in the surfactant/detergent industry.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Chromohalobacter japonicus,Halophilic,Protease,Surfactant-stable[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]This work is funded by Priority Research Grant from Indonesian Ministry of Research and Technology with the contract number of 585u/l1.C01/PL/2016.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]