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Analysis of ethylene biosynthesis gene expression profile during titanium dioxide (TiO2) treatment to develop a new banana postharvest technology
Dwivany F.M.a,b,c, Esyanti R.R.a,c, Suendo V.a, Pratiwi A.S.a, Putri A.A.a,c
a School of Life Science and Technology, Institut Teknologi Bandung, Bandung, 40132, Indonesia
b ForMIND Institute, Bandung, 40132, Indonesia
c Bali International Research Center for Banana, Universitas Udayana, Bukit Jimbaran, Kuta Selatan, Badung, Bali, 80361, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]Copyright © 2019 THE AUTHOR(S).Banana is an important crop that demands proper methods in postharvest handling. As a climacteric fruit, the banana fruit ripening process is affected by ethylene. Several methods have been developed to extend the shelf life of a banana, such as using ethylene scrubbers. In this study, titanium dioxide (TiO2), a photocatalyst, was used as an alternative method to delay the fruit ripening process. The effect of TiO2 on the ripening-related gene MaACS1 was investigated. Banana fruits were placed in a TiO2-coated glass chamber and observed for ten days. Fruit ripening in the treated chamber was delayed for eight days compared to the control. Total RNA was extracted from control and TiO2-treated fruit pulp and synthesized into cDNA. Reverse transcription PCR was performed to investigate the gene expression, which showed that MaACS1 expression was relatively lower than treated control. The finding of these studies suggested that the TiO2 chamber has the potential to extend the shelf life of banana by delaying its ripening process and decreasing the expression of MaACS1. To the best of our knowledge, no previous study has investigated the effect of TiO2 on the expression of genes related to banana fruit ripening.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Banana,Gene expression,Postharvest,Ripening,TiO2[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]This research was partly funded by the Ministry of Research, Technology, and Higher Education of the Republic of Indonesia. The authors thank Banana GroupInstitut Teknologi Bandung for support during this study.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.22146/ijbiotech.51718[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]