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Cloning and in silico studies of lipase gene of a thermophilic bacteria isolated from the hydrothermal vent area of Kawio, North Sulawesi
Lambangsari K.a, Ramdani Moeis M.a, Restiawaty E.a
a School of Life Science and Technology, Institute of Technology Bandung, Bandung, 40132, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2020, Malaysian Society of Applied Biology. All rights reserved.Underwater volcano is an example of a natural habitat of thermophilic bacteria, such as hydrothermal vent Kawio in North Sulawesi. The focus of this research was to screen the bacterial isolates originally from hydrothermal vent Kawio for the production of thermostable lipase. In this study, the lipase gene from the selected isolate was cloned, sequenced, and in silico analysis was performed to predict the characteristics of the lipase protein. One of three isolates examined, showed a positive reaction. Phylogenetic analysis of 16S rRNA gene showed that the isolate was a novel bacteria closely related to Bacillus safensis strain FO-26b with 96,87% homology. Amino acid sequence analysis of the lipase showed similarity between lipase of Bacillus pumilus strain B106 with 98% homology. Further analysis at the protein level indicated that the lipase isolated from marine water was a member of family I.4 of ‘true lipase’ group.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Bacillus safensis,Hydrothermal vent,Lipase,Thermophlic bacteria[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]The authors would like to thank Institute of Technology Bandung for supporting this research.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]