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Isolation and identification of fungi inhabiting rubber-impregnated wood, and their role of quality changing the impregnated wood

Rahniawati N.a, Sumardi I.a, Dungani R.a

a School Life Sciences arid Technology, Bandung Institute of Technology, Bandung, Indonesia

Abstract

© 2020, North Carolina State University.Knowledge of the types of fungi inhabiting wood, their growth conditions, and level of damage is needed to design an effective and efficient preservation process. Researchers have tried to identify fungi that play a role in attacking wood products (impregnated wood), including the type and the role of these fungi in changing the quality of processed wood. Isolation was completed on fungal-attacked wood. The first isolation resulted in approximately 26 isolates, which were then grouped into 8 groups based on the macroscopic appearance of the fungus. Molecular identification of the fungi produced types of Aspergillus tamarii, Penicillium citrinum, Trichoderma longibrachiatum, Trichoderma reesei, and Fusarium solani. Chemical analysis using the Chasson Datta method was performed on attacked and non-attacked wood. A decrease was found in cellulose content and an increase in lignin content for impregnated wood attacked by fungi. It was suspected that there were cellulose-attacking fungi that consumed cellulose, which caused a decrease in cellulose content. An increasing lignin content was thought to be caused by phenols, produced by the delignification process by fungi and additional phenols from the impregnation process. Fourier transform infrared results revealed the presence of carbohydrates, showing that the degradation of cellulose by fungi produced simple carbohydrates.

Author keywords

Fourier transform infra reds,Impregnation process,Isolation and identification,Macroscopic appearance,Molecular identification,Penicillium citrinum,Trichoderma longibrachiatum,Trichoderma reesei

Indexed keywords

Decay wood,FTIR,Identification,Impregnated wood

Funding details

The authors would like to thank the Directorate of Research and the Community Service Directorate General of Research and Development of the Ministry of Research, Technology, and Higher Education (Research Program PUDT 2019) for supporting the implementation of this research.

DOI