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Establishment of hairy root cultures and analysis of saponin production in Javanese ginseng (Talinum paniculatum Gaertn.)
Faizal A.a, Manik V.T.a, Iriawati I.a, Esyanti R.R.a
a Plant Science and Biotechnology Research Group, School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung, 40132, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2020 International Society for Horticultural Science. All rights reserved.The continuous demand for new compounds with important medicinal activities has led to the identification and characterization of various plant-derived natural products. As a part of this program, we report the production of potential pharmaceutical saponin from Talinum paniculatum, widely known as Javanese ginseng. In this research, our results demonstrated the induction of transgenic hairy root lines of T. paniculatum, screening, and selection for saponin production. Two Agrobacterium rhizogenes strains, ATCC 15843 and LBA 9402/12 were used to induce hairy roots following infection of leaf explants from in vitro and ex vitro-grown plants. The effects of bacterial concentration, incubation time, and the presence of acetosyringone were also evaluated respect the efficiency for hairy root induction. Both strains were able to induce hairy roots from leaf explants, but LBA-induced hairy roots showed faster growth rate than ATCC-induced strain. Saponin analysis from hairy root showed that root cultures accumulate higher saponin content compared to non-transgenic roots. Thus, hairy roots are a good material for the production of saponin in T. paniculatum.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Agrobacterium rhizogenes,GFP marker,Medicinal plant,Natural compound[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]The authors acknowledge Indonesian Institute of Sciences and Alain Goossens from VIB-Ghent University, Belgium for providing A. rhizogenes strain ACT 15843 and LBA 9402/12, respectively. This research was partially funded by the Ministry of Research, Technology and Higher Education, the Republic of Indonesia under the scheme of excellent research for university grant (Contract No. 009/SP2H/LT/DRPM/IV/2017).[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.17660/ActaHortic.2020.1285.30[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]