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The effect of glutaraldehyde concentration and drying method on swelling ability and pore size of chitosan-based scaffold
Rusdiputra S.a, Wibowo A.a, Hanifah A.I.a, Wulan Asri L.A.T.a, Purwasasmita B.S.a
a Materials Science and Engineering Research Group, Faculty of Mechanical and Aerospace Engineering, Institut Teknologi Bandung, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2020 American Institute of Physics Inc.. All rights reserved.Corneal damage is the second cause of blindness in the world because of lack of high quality corneal from donor. Chitosan-based scaffold might be attractive for corneal tissue because of their biodegradable and biocompatible properties. In this report, chitosan-based scaffolds were prepared by solution casting combined with salt leaching method and studying the influence of glutaraldehyde (GA) concentration and drying method on swelling ability and pore size of prepared scaffold. Chitosan-based scaffold without addition of GA showed highest swelling ability (31.2 ± 3.8 %) and their swelling ability generally decreased as GA concentration increased. Morphology of scaffold were evaluated by Scanning Electron Microscopy (SEM) method, followed by 3D structure observation using SPIP software and pore size determination using imageJ software. Based on these results, freeze drying method is better than conventional drying method in term of ability to preserve 3D structure and pore of scaffold. Also, addition of GA 0.3% might preserve their 3D structure even though their pore size slightly decreased. Chitosan-based scaffold without addition of GA and dried by freeze drying method has the highest average pore size (274 ± 48 nm).[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]The authors would like to acknowledge the funding provided by Basic Research Fund from Indonesian Ministry for Research, Technology and Higher Education 2019.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.1063/5.0015731[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]