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Nitrogen optimization on rhamnolipid biosurfactant production from pseudoxanthomonas sp. G3 and its preservation techniques Pengoptimuman nitrogen pada pengeluaran biosurfaktan rhamnolipid daripada pseudoxanthomonas sp. G3 dan teknik pemeliharaannya
Purwasena I.A.a, Astuti D.I.a, Utami S.G.a
a Department of Microbiology, School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung, 40132, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2020 Penerbit Universiti Kebangsaan Malaysia. All rights reserved.dispersant, and anti-adhesive. Optimization of biosurfactant production needs to be done, not only to increase its production quantity, but also to reduce overall production cost. This study aims to determine the most suitable and optimum concentration of nitrogen source for biosurfactant production and its preservation techniques. The biosurfactant was produced by Pseudoxanthomonas sp. G3 using minimal salt medium with 2% light crude oil as (IFT), oil drop assay, and dry weight. Potassium sorbate 0.2% (w/v) was used as preservative agent. The results showed that biosurfactant production using sodium nitrate as a nitrogen source provides the highest activity and yield. The E24 value was 76.63% and the clear zone diameter observed was 0.875 cm. The overall decreased in IFT was 35.4% and the biosurfactant dry weight was 0.45 gL-1. Microbial contamination occurred after 3 weeks of storage in the treatment without the addition IFT) were gradually decreased during storage. In conclusion, the optimum biosurfactant production by Pseudoxanthomonas sp. G3 was obtained by adding potassium sorbate which was stored at 4 ℃.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Biosurfactant,Nitrogen,Pottasium sorbate,Preservation,Pseudoxanthomonas sp[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.17576/jsm-2020-4909-10[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]