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Tropical marine Navicula salinicola NBO: Morphology, genetic identification, and biochemical properties
Rachmayanti Y.a, Mwebaza H.a, Radjawane I.M.a, Nurachman Z.a
a Biochemistry Division, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Bandung, 40132, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© Published under licence by IOP Publishing Ltd.Indonesia’s oceans are rich in microalgae as unicellular photosynthetic organisms. So far, most of Indonesia’s diverse microalgae have yet been explored. An Isolate NBO of microalgae has been collected from Bokor Island in the Jakarta Bay, Indonesia. This study aimed to identify the isolate NBO and to characterize its biochemical properties. The identification was carried out based on the physical morphology of cell and the nucleotide sequence of 18S rDNA-V4 and LSU D2/D3 DNA regions, meanwhile, the biochemical properties were characterized based on the content of carbohydrate, lipid and fatty acid of cells. The results showed that the morphological characteristics of NBO cells possessing a color of pale yellow, an oval shape ranging from 11.00-15.00 μm in length and 4.67-5.00 μm in width, and a silica frustule that bilaterally symmetrical oval closed to genus Navicula. Based on the homology of sequences 402-bp of 18S rDNA-V4 and 591-bp of LSU D2/D3, the isolate was identified as Navicula salinicola. Total carbohydrate and lipid contents of Navicula salinicola NBO were 13.13 and 3.74% (w/w), respectively. Fatty acids of Navicula salinicola NBO were dominated by palmitic acid (C16:0), palmitoleic acid (C16:1), and eicosapentaenoic acid (EPA; C20:5) with a total concentration of 38.24, 37.23, and 12.54% (w/w, fatty acid/lipids), respectively, indicating a promising candidate for biodiesel and nutrition/ pharmaceutical.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Biochemical properties,Eicosapentaenoic acid,Genetic identification,Morphological characteristic,Nucleotide sequences,Photosynthetic organisms,Physical morphology,Total carbohydrates[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]We thank the Coordinating Ministry for Maritime and Investment Affairs of the Republic of Indonesia for financial support. Part of the work was funded by the Ministry of Research Technology and Higher Education of Republic Indonesia through the PDUPT Program in 2018.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.1088/1755-1315/618/1/012033[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]