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Gene Expression Related to Steviol Glycoside Synthesis Produced in Stevia rebaudiana (Bert.) Shoot Culture Induced with High Far-Red LED Light in TIS RITA® Bioreactor System
Melviana A.C.a, Esyanti R.R.a, Setyobudi R.H.b, Mel M.c, Adinurani P.G.d, Burlakovs J.e
a School of Life Sciences and Technology, Bandung Institute of Technology, Bandung, 40132, Indonesia
b Department of Agriculture Science, Postgraduate Program, University of Muhammadiyah Malang, Malang, 65145, Indonesia
c Department of Biotechnology Engineering, International Islamic University Malaysia, 53100, Malaysia
d Department of Agrotechnology, Merdeka University of Madiun, Madiun, 63133,, Indonesia
e Department of Water Management, Estonian University of Life Sciences, Tartu, 51014, Estonia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2021. Sarhad Journal of Agriculture. All Rights Reserved.Steviol glycosides contained in Stevia leaves have considerable potential uses for the natural sweetener industry due to it’s antidiabetic properties, hence a large number of plant source is required to supply the industry. To overcome the hindrances in the propagation of Stevia plants using in vivo methods, an alternative technique of propagation using in vitro methods is needed. Te micropropagation method with the RITA® (Recipient for Automated Temporary Immersion System) is used in the production of a large amount of stevia biomass in approximately a short period. Te forming of flowers is one of the limiting factors interfering with the metabolite production, as the content of steviol glycoside will decrease after plant flowering dramatically. Tis mechanism happened because steviol glycoside synthesis and flowering process share the same precursor. However, this interfering factor could be inhibited by using a high-red LED induction to delay the flowering stage, which may contribute to higher biomass and glycoside concentrations through greater system productivity. Hitherto, there was no significant study about the effect of far-red LED induction to improve the steviol glycoside content of Stevia plants, particularly at the molecular level. Tis research was therefore performed in the TIS RITA®bioreactor system to evaluate the effect of far-red LED induction towards biomass growth, multigene expression related to steviol glycosides, and its derivatives, and also the metabolites produced in S. rebaudiana plant. Te result showed that the increment of biomass and gene expressions (ent-KO, ent-KS, ent-KAH13, UGT85C2, UGT74G1, and UGT76G1) in high far-red LED R I TA ®was higher compared with control RITA®. To conclude, the system with a far-red LED induction in TIS RITA® was proven to give a positive efect towards stevia shoots growth and greater metabolites production (stevioside and rebaudioside-A) up to 37.15% and 22.99%, respectively.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Diabetes prevention,Improve the steviol glycoside,In vitro method,Micropropagation,Natural sweetener[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]The authors would like to thank Asahi Glass Foundation for partly funding the research.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.17582/JOURNAL.SJA/2021/37.1.1.8[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]