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Role of Lys59 and Lys386 of streptokinase from type 12 group A streptococcus on in vitro stability against plasmin cleavage
Martius E.a, Mardjuki A.a, Giri-Rahman E.a, Riani C.a, Cahyati Y.a, Retnoningrum D.S.a
a Laboratory of Pharmaceutical Biotechnology, School of Pharmacy, Institut Teknologi Bandung., Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]Streptokinase (SK), is a plasminogen (PG) activator by forming a complex with PG that activates other PG molecule to form plasmin (Pm). In SK from group C streptococci (SKC), the generated Pm immediately inactivates it by cleaving at two major sites, Lys59 and Lys386. The present work is aimed to study whether both residues that are conserved in streptokinase of group A streptococci (SKA), were primary sites for Pm cleavage. Site-dirocted mutagenesis was performed to change both Lys residues to Gin to obtain a mutant SKA (SKA2). The stability of both SKAs was investigated by Western blot analysis in fie presence of PG. The radial caseinolysis and chromogenic assays were performed to evaluate whether the substitutions affected the activity and the kinetic parameters. Our results demonstrated for the first time that the SKA2 was more stable than SKA to Pm cleavage at both Lys residues. The substitutions did not affect the binding affinity to PG, but increased the activity, the catalytic rate, and the catalytic efficiency for PG activation to some extent. This study shows that the processing of Lys59 and Lys386 by Pm is an important event in SKA instability against Pm.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Group A streptoccci,Lys386,Lys59,Pm cleavage,Streptokinase[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]