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In vitro antioxidant activities, total flavonoid, phenolic and carotenoid content from various extracts of four species Asteraceae herb
Fidrianny I.a, Nadia E.a, Komar Ruslan W.a
a School of Pharmacy, Bandung Institute of Technology, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2015, International Journal of Pharmacy and Pharmaceutical Science. All rights reserved.Objectives: The objectives of this research were to study antioxidant activities from various extracts of Asteraceae herbs using two methods of antioxidant assays which were DPPH (2,2-diphenyl-1-picrylhidrazyl) and FRAP (Ferric Reducing Antioxidant Power); and correlation of total flavonoid, phenolic, and carotenoid content in various extracts of Asteraceae herbs with DPPH antioxidant activities and FRAP antioxidant capacities. Methods: Extraction was performed by reflux apparatus using different polarity solvents. The extracts were evaporated using the rotary evaporator. Antioxidant capacities were tested using DPPH and FRAP assays. Determination of total flavonoid, phenolic, and carotenoid content was performed by spectrophotometer UV-visible and their correlation with DPPH antioxidant activities and FRAP antioxidant capacities were analyzed by Pearson’s method. Results: Methanolic extract of Bidens pilosa herbs (BP3) had the highest DPPH scavenging activity with IC5076.25 μg/ml, while ethyl acetate extract of B. pilosa herbs (BP2) had the highest FRAP capacity with EC5033.50 μg/ml. Ethyl acetate extract of B. pilosa (BP2) had the highest total flavonoid (14.66 g QE/100 g), BP3 had the highest phenolic content (7.61 g GAE/100 g), and ethyl acetate extract of Sonchus arvensis (SA2) had the highest carotenoid content (11.92 g BE/100 g). Conclusions: There was a positively high correlation between total phenolic with their antioxidant activity using FRAP and DPPH assays. The FRAP capacities in Artemisia vulgaris, Bidens pilosa, Ageratum conyzoides, and Sonchus arvensis herbs extracts had linear result with DPPH scavenging activities.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Antioxidants,Asteraceae herbs,Carotenoid,DPPH,Flavonoid,FRAP,Phenolic[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]