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Antioxidant capacities from various leaves extracts of four varieties mangoes using DPPH, ABTS assays and correlation with total phenolic, flavonoid, carotenoid
Fidrianny I.a, Rahmiyani I.a, Wirasutisna K.R.a
a School of Pharmacy, Bandung Institute of Technology, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]Objectives: The objectives of this research were to study antioxidant activity of leaves extract from four varieties of mangoes using two methods of antioxidant testing which were DPPH (2.2-diphenyl-1-picrylhydrazyl) and ABTS (2.2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)) and correlation of total phenolic, flavonoid and carotenoid content in various extracts of four varieties mangoes with DPPH and ABTS scavenging acivities. Methods: Extraction was performed by reflux using gradient polarity solvent. The extracts were vaporated using rotavapor. Chromatogram pattern on each extracts were observed by thin layer chromatography (TLC). Then, antioxidant activity of each extracts using DPPH and ABTS assays, IC50 of DPPH and ABTS scavenging activities and determination of total phenolic, flavonoid and carotenoid content and its correlation with DPPH and ABTS scavenging capacities. Results: GD2 (ethyl acetate extract of gedong mangoe leaves) had the highest DPPH scavenging capacity (98.70 %.), while AR3 (ethanolic extract of arumanis mangoe leaves) was the highest ABTS capacity (70.55 %). AR2 (ethyl acetate extract of arumanis mangoe leaves) contained the highest total flavonoid (37.57 g QE/100 g), GD2 had highest phenolic content (30.73 g GAE/100 g), while the highest carotenoid 16.28 g BET/100 g was given by GL1 (n-hexane extract of golek mangoe leaves). Conclusions: There were positively high correlation between total phenolic content in four varieties mangoes leaves with their antioxidant activity using ABTS and DPPH assays. ABTS scavenging activities in various leaves extracts of four varieties mangoes had positively high correlation with their DPPH scavenging activities.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]ABTS,Antioxidants,Carotenoid,DPPH,Flavonoid,Mangoes leaves,Phenolic[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]