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Method development for simultaneous analysis of steroid and non steroid antiinflamatory substances in jamu pegal linu using TLC-spectrophotodensitometry
Aprilia Wisnuwardhani H.a, Fidrianny I.a, Ibrahim S.a
a School of Pharmacy, Bandung Institute of Technology, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]Objective: Illegally added chemical adulteration of jamu become a problem that more difficult to stop. Therefore method development of simultaneous detection of chemical adulteration must be conducted. The aim of this research is to develop analytical method to detect simultaneously chemical adulteration in jamu pegal linu. Methods: Stationary phase, mobile phase, sample preparation and instrument condition were optimized. Results: The result of optimization was validated for specificity according to International Conference on Harmonization. The validated methods were applied to analyze of three jamu pegal linu products in the market for the qualitative analysis of chemical adulteration. Analytical method optimization showed that the best separation of five substances, acetaminophen, dexamethasone, prednisone, mefenamic acid and piroxicam was performed on silica gel GF254, using chloroform – methanol (9:1) as a mobile phase. Samples were prepared by extraction using ethanol as a solvent. Extraction was done for 30 minutes using 3D shaker. Chamber saturation was done for 60 minutes. Detection of chemical adulteration was performed using CAMAG TLC (Thin Layer Chromatography) Scanner at λ 254 nm for simultaneous detection of acetaminophen, dexamethasone, prednisone, mefenamic acid, piroxicam. Conclusion: A new validated methods for qualitative analysis (acetaminophen, dexamethasone, prednisone, mefenamic acid and piroxicam was deeloped) by thin layer chromatography (TLC) at λ 254 nm. The separation was performed on silica gel GF254, using chloroform – methanol (9:1) as mobile phase. The methods can be used to analyze jamu pegal linu in the market.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Jamu pegal linu,Simultaneous detection,TLC-spectrophotodensitometry[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]