[vc_empty_space][vc_empty_space]
Biodesulfurization of subbituminous coal by mixed culture bacteria isolated from coal mine soil of South Sumatera
Aditiawati P.a, Akhmalokaa, Astuti D.I.a, Sugilubina, Pikoli M.R.a,b
a Microbial Biotechnology Research Division, School of Life Science and Technology, Institute Teknologi Bandung, Indonesia
b Department of Biology, Faculty of Science and Technology, State Islamic University Syarif Hidayatullah Jakarta, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]Coal as fuel should be necessarily pre-treated by desulfurization in order to prevent excessive emissions of sulfur dioxide, a precursor of acid rain. Organic sulfur in coal can be eliminated by microbial action through the technology known as biodesulfurization. Source of microorganisms in the present study was coal mine soil in which microorganisms have been adapted to use the sulfur in coal. Coal mine in South Sumatra was choosen as source of microorganisms in this study, because it is an area in Indonesia with the largest of subbituminous coal reserves. The microorganisms were activated as mixed culture by culturing the soil sample in mineral salt medium containing subbituminous coal as the sole sulfur. Desulfurization activities were examined by using three variations of the initial coal concentration, i.e., 10, 15 and 20% weight per volume. Growth and activity of the mixed culture on the subbituminous coal were monitored by measuring of medium pH, cell concentration, sulfate and organic sulfur concentration. The result showed that desulfurization activity of the mixed culture on 15% of coal was able to reduce sulfur up to 82.36%. Isolation and identification of the mixed culture based on genotypic and phenotypic characterizations revealed that members of the mixed culture were identified as genera of Enterobacter, Lelcersia and Bacillus. Observation on growth curves showing that the culturable isolates grew in at least three overlapping stages when using coal as sulfur source suggested that the members of the consortium worked alternately on coal as substrate. © 2013 Asian Network for Scientific Information.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Biodesulfurization,Isolation and identification,Mineral salt mediums,Mixed cultures,Mixed-culture bacteria,Organic sulfur,Phenotypic characterization,Subbituminous coal[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Biodesulfurization,Mixed culture,Organic sulfur,Subbituminous coal[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.3923/biotech.2013.46.53[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]