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The existence of gene deoxyribonucleic acid polymerase mutation from positive hepatitis B samples in bandung, Indonesia
Rostinawati T.a, Retnoningrum D.S.b, Pratiwi D.a
a Department of Biological Pharmacy, Pharmaceutics, Faculty of Pharmacy, Universitas Padjadjaran, Indonesia
b Department of Biological Pharmacy, Pharmaceutics, School of Pharmacy, Institut Teknologi Bandung, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2017 The Authors.Objective: The objective of the study is to complete mutation existence in reverse transcriptase (RT) domain of the viral polymerase. Methods: The study was done by amplification step of viral polymerase gene fragment, agarose gel electrophoresis, polymerase chain reaction product purification with GFX column kit, sequencing, and sequencing result analysis. The samples were derived from clinical laboratory in Bandung Indonesia. Results: The result showed mutations in deoxyribonucleic acid (DNA) fragment encoding for RT domain viral polymerase in sample 6, 7, and 8. There were mutations leading to amino acid substitution L526S in sample 6, D551E in sample 7, and D552E in sample 8. Conclusion: D551E and D552E substitution occurred in YMDD motif RT DNA polymerase that produced YMDE mutant. L526S, D551E, and D552E were estimated as antivirus-resistance mutants that have never been reported before.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Gene encoding for reverse transcriptase deoxyribonucleic acid polymerase,Hepatitis B virus,Mutation[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.22159/ajpcr.2017.v10s2.19493[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]