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A novel immune-tolerable and permeable lectin-like protein from mushroom Agaricus bisporus
Ismaya W.T.a, Yunitab, Efthyani A.b, Lai X.c,d, Retnoningrum D.S.b, Rachmawati H.b, Dijkstra B.W.c,d, Tjandrawinata R.R.a
a Dexa Laboratories of Molecular Sciences, JABABEKA II Industrial Estate, Cikarang, 17550, Indonesia
b Research Group of Pharmaceutics, School of Pharmacy, Bandung Institute of Technology, Bandung, 40132, Indonesia
c European Synchrotron Radiation Facility, Grenoble, 38000, France
d Laboratory of Biophysical Chemistry, University of Groningen, Groningen, 9747AG, Netherlands
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2016 Elsevier Inc. All rights reserved.A lectin like protein designated as LSMT is recently discovered in Agaricus bisporus. The protein adopts very similar structure to Ricin-B like lectin from Clitocybe nebularis (CNL) and HA-33 from Clostridium botulinum (HA-33), which both recognize sugar molecules that decorate the surface of the epithelial cells of the intestine. A preliminary study in silico pointed out potential capability of LSMT to perform such biological activity. Following that hypothesis, we demonstrated that LSMT is indeed capable of penetrating out from a dialysis tube of the mice intestine origin. Furthermore, the protein appeared not to evoke the immune response upon introduction into mice, unlike its structural homologs. This is the first report on the biological implication of LSMT that might lead to its application.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Animals,Computer Simulation,Female,Immune Tolerance,Intestinal Absorption,Lectins,Mice,Models, Chemical,Models, Immunological,Permeability,Sequence Analysis, Protein,Sequence Homology, Amino Acid[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Agaricus bisporus lectin-like protein,Mushroom tyrosinase light subunit,ORF239342[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]This work was supported by the Ministry of Research, Science, Technology, and Higher Education, Republic of Indonesia through Excellent Research Program 2015–2018 (Contract No. 1059.10/I1.C03/KU/2015 ) and Dexa Laboratories of Molecular Sciences, Dexa Medica .[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.1016/j.bbrc.2016.04.020[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]