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Cotton fiber-based assay with time-based microfluidic absorption sampling for point-of-care applications
Wicaksono D.H.B.a, Syazwani I.N.b, Ratnarathorn N.c, Sadir S.b, Shahir S.b, Ruckthong L.c, Dungchai W.c, Yuliarto B.d, Dipojono H.K.d
a Department of Biomedical Engineering, Faculty of Life Science and Technology, Swiss German University (SGU), Tangerang, Banten, 15143, Indonesia
b Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, UTM, Skudai, Johor, 81310, Malaysia
c Organic Synthesis, Electrochemistry and Natural Product Research Unit, Department of Chemistry, Faculty of Science, King Mongkut’s University of Technology Thonburi (KMUTT), Thungkru, Bangkok, 10140, Thailand
d Department of Engineering Physics, Institut Teknologi Bandung (ITB), Bandung, 40132, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2019 Newlands Press.Aim: Time-based microfluidic absorption sampling was proposed using cotton fiber-based device made in swab stick. The assay was optimized and compared with conventional pipetted drop sampling using the same device. Materials & methods: Reagents were integrated into cotton fiber device for assessing concentration of analytes by the colorimetric detection method through time-based absorption sampling microfluidic system. All assay parameters were first optimized using conventional pipette-based drop sampling. Results: The color intensity is linear in the relevant concentration range of the analytes. The LOD are 0.189 mM for glucose and 6.56 μM for nitrite, respectively. These values are better than conventional drop sampling. The fiber-containing swab itself functions as sampling, assay and calibration device. Conclusion: Microfluidic cotton fiber-based assay device was fabricated and can determine analyte concentration in artificial salivary samples, colorimetrically, by time-based absorption sampling without the need of complex equipments.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Absorption, Physicochemical,Cotton Fiber,Food Coloring Agents,Lab-On-A-Chip Devices,Point-of-Care Systems,Time Factors[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Absorption sampling,Colorimetric sensing,Cotton,Fiber-based assay,Glucose,Microfluidic,Nitrite,Noninvasive diagnostic[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]The project is partially funded by Innovation and Commercialization Centre of Universiti Teknologi Malaysia (UTM) under Vot. No. Q J140000 22B3 00C108; Thailand Research Fund (TRF) through MRG6080171 and Post-doctoral Fellowship, Research Innovation and Partnerships Office, King Mongkut’s University of Technology Thonburi (KMUTT) and Swiss German University’s (SGU) Central Research Fund (CRF) on Flexible Microfluidic Device, as well as by the research grant from: Indonesian Ministry of Research, Technology, and Higher Educations; World Class Professor research grant and also ITB research grant program. The authors thank UTM’s Innovation and Commercialization Centre (ICC), TRF and SGU’s Academic Research and Community Service (ARCS) centre and ITB’s Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) for managing the grants. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.4155/bio-2018-0190[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]