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Design of smart nano device for intracellular targeting
Mudhakir D.a, Tan E.b, Akita H.b, Harashima H.b
a School of Pharmacy, Bandung Institute of Technology (ITB), Indonesia
b Faculty of Pharmaceutical Sciences, Hokkaido University, Japan
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]To obtain an efficient delivery of macromolecule such as siRNA, a sophisticated design of vector is needed. It must be retained to the active uptake by reticuloendothelial system (RES) in the bloodstream and it can release its content inside the cellular target site. In this study, we attempted to design a vector which accommodates the needs. siRNA was encapsulated in the liposomes and its surface was shielded with polyethylene glycol (PEG) moiety to avoid recognizing by RES system. To facilitate cellular internalization, newly synthetizing peptide was attached to tip end of PEG moiety. As results, blood concentration of PEG-modified liposomes was higher than that of unmodified PEG. Results by confocal laser scanning microscopy studies exhibited PEG-coated nanodevice can escape from endosomes in both two types PEG used, shorter and longer one. However, prominent cytosolic release of siRNA was only shown by the use of shorter PEG type. These results were in line with gene silencing study in which shorter PEGylated nanostructure achieved dramatic levels of transgene expression study. In conclusion, shorter PEG modified-liposomes encapsulating siRNA could be utilized as a device for in vivo use. © 2011 American Institute of Physics.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]intracellular trafficking,liposomes,PEGylation,siRNA,transgene knockdown[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.1063/1.3667260[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]