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Analysis of electropherogram profile of crude cartilage oligomeric matrix protein for rheumatoid arthritis diagnosis
Saptarini N.M.a,b, Wibowo M.S.a, Ihsanawatia, Gusdinar T.a
a Department of Pharmacochemistry, School of Pharmacy Institut Teknologi Bandung, West Java, 40132, Indonesia
b Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, West Java, 45363, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2017 Nyi Mekar Saptarini et al.Cartilage oligomeric matrix protein (COMP) is a potential biomarker to monitor the development of cartilage damage and injury in rheumatoid arthritis (RA). The most accurate method of determining COMP levels is the enzyme-linked immunosorbent assay, but this method is expensive and requires trained analysts. An alternative method to solve this problem is analyzing the electropherogram profile of crude COMP. This method separates proteins based on molecular weight, so it can differentiate pentamer COMP from its fragments. This study was conducted to analyze the electroferogram profile of crude COMP of RA patients and normal individuals, so it can be applied to RA diagnosis. COMP was precipitated on its isoelectric pH, then crude COMP was dissolved and electrophoresed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE).The electropherogram profiles of RA patients were dominated by pentamer and dimerbands, whereas the electropherogram profiles of normal individual were dominated by tetramer, trimer, and dimer bands. Increased pentamer COMP in serum was indicated by the cartilage damage. The electropherogram profile of crude COMP of RA patient can be differentiated from normal individuals, so it can be used for the RA diagnosis.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Crude COMP,Isoelectricp H,Precipitation,SDS-PAGE,Serum[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.7324/JAPS.2017.71221[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]