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Effect of cryoprotectants on sperm vitrification
Widyastuti R.a, Lesmana R.a, Boediono A.b, Sumarsono S.H.c
a Laboratory of Animal Reproduction and Artificial Insemination, Department of Animal Production, Animal Husbandry Faculty, Universitas Padjadjaran, Sumedang, Indonesia
b Laboratory of Embryology, Department of Anatomy, Physiology and Pharmacology, Faculty of Veterinary Medicine, Institute of Bogor Agriculture, Jl. Agatis Dramaga Bogor, Indonesia
c Physiology Developmental Biology and Biomedical Science Research Group, School of Life Science and Technology, Bandung Institute of Technology, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2017 Taylor & Francis Group, London.One of the problems of using high concentrations of cryoprotectants for sperm vitrification is the cytotoxic effect that affects sperm recovery after warming. Therefore, in this study, we determined the recovery rate after vitrification with and without cryoprotectants. Ejaculates with progressive motility and viability above 50% were used as samples. The samples were divided into two groups: (1) samples were mixed with a basic solution (2) samples were mixed with a vitrification solution. Sperm were vitrified by direct plunging into LN2. Sperm motility and viability were observed to evaluate the quality of sperm before and after vitrification. Overall, the sperm samples vitrified with cryoprotectants had a significantly higher proportion of sperm motility (56%) and viability (58.15%) compared with those vitrified without cryoprotectants (35% and 48%, respectively, p < 0.05). However, vitrification of human sperm without cryoprotectants could be recommended for routine assisted reproductive technology.[/vc_column_text][vc_empty_space][vc_separator css=".vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}"][vc_empty_space][megatron_heading title="Author keywords" size="size-sm" text_align="text-left"][vc_column_text]Assisted reproductive technologies,Basic solutions,Cryoprotectants,Cytotoxic effects,Human sperm,Recovery rate,Sperm motility,Sperm viability[/vc_column_text][vc_empty_space][vc_separator css=".vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}"][vc_empty_space][megatron_heading title="Indexed keywords" size="size-sm" text_align="text-left"][vc_column_text]Cryoprotectants,Sperm motility,Sperm viability,Sperm vitrification[/vc_column_text][vc_empty_space][vc_separator css=".vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}"][vc_empty_space][megatron_heading title="Funding details" size="size-sm" text_align="text-left"][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=".vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}"][vc_empty_space][megatron_heading title="DOI" size="size-sm" text_align="text-left"][vc_column_text]https://doi.org/10.1201/9781315208619-27[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]