2-s2.0-84991503878

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[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2016 Lida Amalia et al.Background: Chromium (Cr) plays a role in the production of Reactive Oxygen Species (ROS) that cause oxidative stress. Oxidative stress is a condition when the amount of ROS, such as H2O2, and OH-2- (as a by products in a number of metabolic reactions in the cell organelles) exceeds the amount of antioxidants produced. Antioxidants can be either enzyme or non-enzyme. Antioxidant enzymes are such as catalase and ascorbate peroxidase. In the field, it was found that bananas cv. ambon lumut that can be grown in Cr polluted areas. In this study, it was observed that the level expression of catalase (CAT) and ascorbate peroxidase (APX) genes on banana plantlets cv. ambon lumut to determine the mechanisms of banana plants under Cr stress conditions. Materials and Methods: Banana plantlets were grown in vitro on MS medium with the treatment of Cr at 0, 50, 100, 200 and 400 ppm concentrations and maintained for 6 weeks. Growth parameters were analyzed by ANOVA at significance level (α) of 0.05. Gene expression level can be undertaken with qRT-PCR analysis approach. Results: The growth rate of banana plantlets decreased with increasing Cr concentrations in the growth mediums, especially at 200 and 400 ppm. The level of CAT and APX genes expression were observed in the roots and shoots of banana plantlets. At the root, CAT and APX genes are expressed in all treatment conditions, except in the treatment of 50 ppm. At the shoot, only CAT gene was expressed higher than the control and the highest level of CAT gene expression occurred in the treatment of 200 ppm. The level of CAT and APX genes expression reached the highest level on the treatment of 200 ppm. The level of gene expression of CAT reached more than 9 times compared to the control and APX more than three times. Conclusion: (1) The growth rate of banana plantlets decreased with increasing Cr concentrations in the growth mediums and (2) The level of CAT and APX gene expression in plantlets under Cr stress condition were higher than the control. Gene expression level in the roots is higher than the shoots.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Ascorbate peroxidase,Catalase,Chromium stress,Gene expression level,Growth,Musa acuminata[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.3923/jps.2016.69.74[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]