[vc_empty_space][vc_empty_space]
Development of transdermal nanoemulsion formulation for simultaneous delivery of protein vaccine and artin-m adjuvant
Suciati T.a, Aliyandi A.a, Satrialdia
a School of Pharmacy, Bandung Institute of Technology, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]Objective: Vaccine used to enhance immune response is commonly administered via parenteral route. However, it shows some disadvantages. This research aimed to develop a non-invasive vehicle for administering an immunogenic protein and a lectin adjuvant via transdermal route. The hydrophilic nature of protein and lectin became the major challenge faced in this study. Methods: Bovine serum albumin (BSA) was used as protein model and artin-M isolated from cempedak (Artocarpus integrifolia) seed as adjuvant model. Nanoemulsion was formulated as oil in water type and prepared by self-nanoemulsification method. The formulation consisted of virgin coconut oil (VCO), Tween 80, and PEG 400 as oil phase, surfactant, and co-surfactant, respectively. BSA and artin-M were incorporated into the oil phase as co-lyophilized solid dispersion in PEG 20000. The nanoemulsion was characterized by BSA entrapment efficiency, hemagglutination activity of artin-M, and stability testing using freeze thaw conducted by alternating the storage at room temperature and 4 °C up to 6 cycles. Results: The addition of PEG 20000 in solid dispersion at the rasio of 1:1 to BSA markedly increased BSA entrapment efficiency in oil phase to 98.6 ± 0.15%. Artin-M incorporated using the same method was also maintained its activity as shown by hemagglutination testing. The freeze thaw testing revealed the stability of nanoemulsion stored in the fridge with no-aggregation observed. The globule diameter was maintained at the size of 42.7 ± 0.9 nm with polydispersity index of 0.321 ± 0.01.Conclusion: The nanoemulsion is potensial as simultaneous transdermal delivery of protein vaccine and lectin adjuvant.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Artin-M,BSA,Nanoemulsion,Transdermal,Vaccine[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]