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Effect of calcium ions to the activity and stability of extracellular lipase produced by moderate halophilic bacteria Halomonas meridiana BK-AB4
Nanda G.A.M.a, Hertadi R.a
a Department of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Bandung, 40132, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2020, Malaysian Society of Applied Biology. All rights reserved.This study reported the isolation and biochemical studies of extracellular lipase produced by a moderate halophilic bacterium, Halomonas meridiana BK-AB4. The estimated molecular weight of the lipase BK-AB4 is about 50 kDa. The study of metal ions effect to the enzyme activity revealed the strong dependency towards the presence of Ca2+ ion. Further studies showed that the optimum pH of the enzyme was similar in the presence and the absence of Ca2+ ions. However, the presence of Ca2+ ions could improve the enzyme thermal stability as indicated by the shift of its optimum temperature from 45°C-50°C. The addition of Ca2+ ions was also enhancing residual activity towards various inhibitors, such as SDS and PMSF up to 55% and 68%, respectively. Another interesting effect of the addition of Ca2+ ions were noted when various organic solvents were added to the enzyme solution in the ratio of 1:1. The activity of the enzyme increased up to 122% and 126% relative to the control when methanol and n-butanol were added, respectively. This study thus showed that the addition of Ca2+ ion not only improved the catalytic activity of lipase BK-AB4 but also made the enzyme more tolerable against various perturbations.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Extracellular lipase,Halomonas meridiana BK-AB4,Halophilic bacteria,Metal ions activator[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]